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FUNCTIONAL GAIN OF FRUIT NETTED-CRACKING IN AN INTROGRESSION LINE OF TOMATO WITH HIGHER EXPRESSION OF

《农业科学与工程前沿（英文）》 2021年 第8卷 第2期

摘要：

Fruit cracking is a major disorder that affects the integrity of fruit and reduces the commercial value of tomato and other fleshy fruit. Here, we have found a novel fruit ‘netted-cracking’ (FNC) phenotype in tomato introgression line IL4-4 which is present in neither the donor parent (LA0716) nor the receptor parent (M82). An F2 population was generated by crossing IL4-4 with M82 to genetically characterize the FNC gene and this showed that a single dominant gene determined fruit netted-cracking. Further map-based cloning narrowed down the FNC locus to a 230 kb region on chromosome 4. Sequencing and annotation analysis show that FNC(Solyc04 g082540) was the most likely candidate gene. Functional characterization of FNC by overexpressing FNCAC and FNCIL4-4resulted in the fruit netted-cracking phenotype, suggesting that the FNC transcript level results in the functional gain of fruit netted-cracking. These findings were further confirmed by FNC ortholog in netted-cracking pepper and melon, indicating a common regulatory mechanism in different plant species. Furthermore, cytoplasm and nucleus-localized FNC indicates increased expression of genes involved in suberin, lignin, lipid transport and cell wall metabolism. These findings provide novel genetic insights into fruit netted-cracking and offer a way to promote molecular improvement toward cracking resistant cultivars.

 

关键词： fine mapping / fruit netted-cracking / introgression line / transcript level    

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FUNCTIONAL GAIN OF FRUIT NETTED-CRACKING IN AN INTROGRESSION LINE OF TOMATO WITH HIGHER EXPRESSION OF

Chunli ZHANG, Taotao WANG, Jing LI, Danqiu ZHANG, Qingmin XIE, Shoaib MUNIR, Jie YE, Hanxia LI, Yongen LU, Changxian YANG, Bo OUYANG, Yuyang ZHANG, Junhong ZHANG, Zhibiao YE

《农业科学与工程前沿（英文）》   页码 280-291 doi: 10.15302/J-FASE-2020374

摘要： Fruit cracking is a major disorder that affects the integrity of fruit and reduces the commercial value of tomato and other fleshy fruit. Here, we have found a novel fruit ‘netted-cracking’ (FNC) phenotype in tomato introgression line IL4-4 which is present in neither the donor parent (LA0716) nor the receptor parent (M82). An F population was generated by crossing IL4-4 with M82 to genetically characterize the gene and this showed that a single dominant gene determined fruit netted-cracking. Further map-based cloning narrowed down the locus to a 230 kb region on chromosome 4. Sequencing and annotation analysis show that (Solyc04 g082540) was the most likely candidate gene. Functional characterization of by overexpressing and resulted in the fruit netted-cracking phenotype, suggesting that the transcript level results in the functional gain of fruit netted-cracking. These findings were further confirmed by ortholog in netted-cracking pepper and melon, indicating a common regulatory mechanism in different plant species. Furthermore, cytoplasm and nucleus-localized indicates increased expression of genes involved in suberin, lignin, lipid transport and cell wall metabolism. These findings provide novel genetic insights into fruit netted-cracking and offer a way to promote molecular improvement toward cracking resistant cultivars.

关键词： fine mapping     fruit netted-cracking     introgression line     transcript level    

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Acoustic fault signal extraction via the line-defect phononic crystals

《机械工程前沿（英文）》 2022年 第17卷 第1期   页码 10-10 doi: 10.1007/s11465-021-0666-y

摘要： Rotating machine fault signal extraction becomes increasingly important in practical engineering applications. However, fault signals with low signal-to-noise ratios (SNRs) are difficult to extract, especially at the early stage of fault diagnosis. In this paper, 2D line-defect phononic crystals (PCs) consisting of periodic acrylic tubes with slit are proposed for weak signal detection. The defect band, namely, the formed resonance band of line-defect PCs enables the incident acoustic wave at the resonance frequency to be trapped and enhanced at the resonance cavity. The noise can be filtered by the band gap. As a result, fault signals with high SNRs can be obtained for fault feature extraction. The effectiveness of weak harmonic and periodic impulse signal detection via line-defect PCs are investigated in numerical and experimental studies. All the numerical and experimental results indicate that line-defect PCs can be well used for extracting weak harmonic and periodic impulse signals. This work will provide potential for extracting weak signals in many practical engineering applications.

关键词： phononic crystals     line-defect     fault signal extraction     acoustic enhancement    

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Calculations of narrow-band transimissities and the Planck mean absorption coefficients of real gases using line-by-line

Huaqiang CHU, Mingyan GU, Huaichun ZHOU, Fengshan LIU

《能源前沿（英文）》 2014年 第8卷 第1期   页码 41-48 doi: 10.1007/s11708-013-0292-4

摘要： Narrow-band transmissivities in the spectral range of 150 to 9300 cm and at a uniform resolution of 25 cm were calculated using the statistical narrow-band (SNB) model with the band parameters of Soufiani and Taine, the more recent parameters of André and Vaillon, and the line-by-line (LBL) method along with the HITEMP-2010 spectroscopic database. Calculations of narrow-band transmissivity were conducted for gas columns of different lengths and containing different isothermal and non-isothermal CO -H O-N mixtures at 1 atm. Narrow-band transmissivities calculated by the SNB model are in large relative error at many bands. The more recent SNB model parameters of André and Vaillon are more accurate than the earlier parameters of Soufiani and Taine. The Planck mean absorption coefficients of CO , H O, CO, and CH in the temperature range of 300 to 2500 K were calculated using the LBL method and different versions of the high resolution transmission (HITRAN) and high-temperature spectroscopic absorption parameters (HITEMP) spectroscopic databases. The SNB model was also used to calculate the Planck mean absorption coefficients of these four radiating gases. The LBL results of the Planck mean absorption coefficient were compared with the classical results of Tien and those from the SNB model.

关键词： transimissity     HITEMP     HITRAN     Planck mean absorption coefficients    

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Pareto lexicographic α-robust approach and its application in robust multi objective assembly line balancing

null

《机械工程前沿（英文）》 2014年 第9卷 第3期   页码 257-264 doi: 10.1007/s11465-014-0294-x

摘要：

Robustness in most of the literature is associated with min-max or min-max regret criteria. However, these criteria of robustness are conservative and therefore recently new criteria called, lexicographic α-robust method has been introduced in literature which defines the robust solution as a set of solutions whose quality or jth largest cost is not worse than the best possible jth largest cost in all scenarios. These criteria might be significant for robust optimization of single objective optimization problems. However, in real optimization problems, two or more than two conflicting objectives are desired to optimize concurrently and solution of multi objective optimization problems exists in the form of a set of solutions called Pareto solutions and from these solutions it might be difficult to decide which Pareto solution can satisfy min-max, min-max regret or lexicographic α-robust criteria by considering multiple objectives simultaneously. Therefore, lexicographic α-robust method which is a recently introduced method in literature is extended in the current research for Pareto solutions. The proposed method called Pareto lexicographic α-robust approach can define Pareto lexicographic α-robust solutions from different scenarios by considering multiple objectives simultaneously. A simple example and an application of the proposed method on a simple problem of multi objective optimization of simple assembly line balancing problem with task time uncertainty is presented to get their robust solutions. The presented method can be significant to implement on different multi objective robust optimization problems containing uncertainty.

关键词： Pareto     lexicographic α-robust     assembly line balancing    

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Choosing configurations of transmission line tower grounding by back flashover probability value

Dmitry KUKLIN

《能源前沿（英文）》 2016年 第10卷 第2期   页码 213-226 doi: 10.1007/s11708-016-0398-6

摘要： There is a considerable number of works devoted to electrical characteristics of grounding. These characteristics are important in general. However, in application to grounding of transmission line towers they are not enough to determine what grounding construction is preferable in some particular case, because these characteristics are calculated or measured apart from the grounded object, and only limited number of current (or voltage) source waveforms is used. This paper indicates reasons in favor of the fact that to choose the optimum design of grounding, the calculation model should include the tower as it is. The probability of back flashover, which provides both qualitative and quantitative estimate of the grounding structure efficiency, can be taken as the criterion for the grounding design. The insulation flashover probability is calculated on the basis of engineering method, which evaluates breakdown strength of insulation for nonstandard waveshapes, and probability data on lightning currents. Different approaches are examined for identifying the back flashover probability, as not only amplitudes but also other parameters can be taken into account. Finite-difference time-domain method is used for calculations of transients. It is found that lightning current waveform can greatly influence calculated back flashover probability value.

关键词： grounding     transmission line tower     back flashover probability     FDTD method    

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A fast compound direct iterative algorithm for solving transient line contact elastohydrodynamic lubrication

null

《机械工程前沿（英文）》 2014年 第9卷 第2期   页码 156-167 doi: 10.1007/s11465-014-0297-7

摘要：

A fast compound direct iterative algorithm for solving transient line contact elastohydrodynamic lubrication (EHL) problems is presented. First, by introducing a special matrix splitting iteration method into the traditional compound direct iterative method, the full matrices for the linear systems of equations are transformed into sparse banded ones with any half-bandwidth; then, an extended Thomas method which can solve banded linear systems with any half-bandwidth is derived to accelerate the computing speed. Through the above two steps, the computational complexity of each iteration is reduced approximately from O(N3/3) to O(β2N), where N is the total number of nodes, and β is the half-bandwidth. Two kinds of numerical results of transient EHL line contact problems under sinusoidal excitation or pure normal approach process are obtained. The results demonstrate that the new algorithm increases computing speed several times more than the traditional compound direct iterative method with the same numerical precision. Also the results show that the new algorithm can get the best computing speed and robustness when the ratio, half-bandwidth to total number of nodes, is about 7.5%–10.0% in moderate load cases.

关键词： elastohydrodynamic lubrication     transient     line contact     matrix splitting iteration method     the Thomas method    

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A novel light fluctuation spectrum method for in-line particle sizing

Shouxuan QIN, Xiaoshu CAI, Li MA

《能源前沿（英文）》 2012年 第6卷 第1期   页码 89-97 doi: 10.1007/s11708-012-0176-z

摘要： This paper discusses two problems in in-line particle sizing when using light fluctuation method. First, by retrieving the ratio of particle concentrations at different time, the intensity of incident light is obtained. There exists narrow error between the calculated and pre-detected value of the intensity of incident light. Secondly, by combining spectrum analysis with Gregory’s theory, a multi-sub-size zone model is proposed, with which the relationship between the distribution of turbidity and the particle size distribution (PSD) can be established, and an algorithm developed to determine the distribution of turbidity. Experiments conducted in the laboratory indicate that the measured size distribution of pulverized coal conforms well with the imaging result.

关键词： in-line measurement     particle size distribution (PSD)     incident light intensity     particle concentration     light fluctuation    

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A naive optimization method for multi-line systems with alternative machines

Weichang KONG, Fei QIAO, Qidi WU

《机械工程前沿（英文）》 2019年 第14卷 第4期   页码 377-392 doi: 10.1007/s11465-019-0544-z

摘要： The scheduling of parallel machines and the optimization of multi-line systems are two hotspots in the field of complex manufacturing systems. When the two problems are considered simultaneously, the resulting problem is much more complex than either of them. Obtaining sufficient training data for conventional data-based optimization approaches is difficult because of the high diversity of system structures. Consequently, optimization of multi-line systems with alternative machines requires a simple mechanism and must be minimally dependent on historical data. To define a general multi-line system with alternative machines, this study introduces the capability vector and matrix and the distribution vector and matrix. A naive optimization method is proposed in accordance with classic feedback control theory, and its key approaches are introduced. When a reasonable target value is provided, the proposed method can realize closed-loop optimization to the selected objective performance. Case studies are performed on a real 5/6-inch semiconductor wafer manufacturing facility and a simulated multi-line system constructed on the basis of the MiniFAB model. Results show that the proposed method can effectively and efficiently optimize various objective performance. The method demonstrates a potential for utilization in multi-objective optimization.

关键词： multi-line systems     alternative machines     feedback control     closed-loop optimization    

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Establishment and drug sensitivity evaluation of murine ascites hepatocarcinoma cell line with high lymphatic

Hongying ZHANG, Jianwu TANG, Wenting ZHU, Chunxiu HU, Guowang XU

《医学前沿（英文）》 2009年 第3卷 第2期   页码 119-129 doi: 10.1007/s11684-009-0022-9

摘要： In order to provide a sensitive cell line model for investigating the mechanisms underlying the lymphatic metastasis of tumors and the effect of medicine against cells, a new murine ascites hepatocarcinoma cell line with high lymphatic metastatic potential (Hca-P/L ) was established and the effect of curcumin on biological behavior of Hca-P/L was observed. Murine ascites hepatocarcinoma cell strain with low lymphatic metastatic potential (Hca-P) was subcutaneously inoculated into the medioventral line of a mouse 615 and the first generation of metastatic tumor cells of inguinal lymph node (Hca-P/L ) was obtained. Then, Hca-P/L was screened by the route of mouse foot pad subcutaneously → lymph node → scale-up culture → mouse foot pad subcutaneously for five times consecutively. The sensitivity of two murine ascites hepatocarcinoma cell lines (Hca-P and Hca-P/L ) and two anchorage-dependent human hepatocarcinoma cell lines (SMC7721 and HepG ) to curcumin were studied by use of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay after these cells had been pretreated by curcumin at the concentration of 15-240 μmol/L for 48 h. After pretreatment by curcumin at the maximum non-cytotoxic dose of 15 μmol/L , the effect of curcumin against cell proliferation of Hca-P and Hca-P/L was observed by inverted microscope, cell growth curve and cell population doubling time; the effects of curcumin on cell cycles of Hca-P/L and Hca-P were studied by flow cytometry (FCM). The results showed Hca-P/L spreading to the lymph nodes at multiple sites in mice was screened from Hca-P. The lymph node metastatic rate was 100%. Curcumin had significant growth inhibiting effect on both murine ascites and human hepatocarcinoma cell lines in a dose-dependent manner ( <0. 05). At concentrations of 30-120 μmol/L, curcumin had more inhibition on murine ascites hepatocarcinoma cell lines than on human anchorage-dependent hepatocarcinoma cell lines. At concentrations of 60-240 μmol/L, curcumin had more inhibition on Hca-P/L with (the 50% inhibitory concentration) IC of 51.48 μmol/L than on Hca-P with IC of 90.87 μmol/L. After pretreatment by curcumin at the maximum non-cytotoxic dose of 15 mol/L for 7 days, the proliferations of Hca-P/L and Hca-P were inhibited ( <0.05) in a time-dependent manner ( <0.01) and the population doubling time of Hca-P/L and Hca-P was prolonged ( <0.01), and curcumin had more inhibition on Hca-P/L than on Hca-P ( <0.05). After pretreatment by 15 μmol/L curcumin for 48 h, the morphous of Hca-P/L was influenced more seriously than that of Hca-P and the cell cycle was redistributed with Hca-P/L being blocked in the S phase and Hca-P in the S and G /M phases. Hca-P/L was validated to be more sensitive to curcumin than Hca-P. Hca-P/L is a novel sensitive cell line model for investigating the mechanisms underlying tumor lymphatic metastasis and the effect of the medicine against cells.

关键词： murine ascites hepatocarcinoma cell line     metastasis     curcumin     drug sensitivity    

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基于灰色模糊数的公交线网优化研究

邓卫,胡启洲

《中国工程科学》 2007年 第9卷 第11期   页码 21-25

摘要：

在考虑信息不完全的情况下，用灰色模糊数对公交线网优化问题进行了研究。在给出了公交线网优化的约束条件与优化目标的基础上，利用灰色模糊数建立了公交线网优化的决策模型。灰色模糊数是用三参数区间参与优化过程，在允许参数在一定范围内变化的情况下，计算后得到的结果是一个区间向量，其中向量值最大的为所求结果，适用于城市公交线网的优化问题。实例应用分析表明，优化后的公交线网效率提高，可达性良好，可满足城市公交可持续发展的要求。

关键词： 公交线网     优化     灰色模糊数    

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Performance analysis for a two-way relaying power line networkwith analog network coding

Yu-wen QIAN,Meng TIAN,Xue JIANG,Hua-ju SONG,Feng SHU,Jun LI

《信息与电子工程前沿（英文）》 2015年 第16卷 第10期   页码 892-898 doi: 10.1631/FITEE.1500135

摘要： In this paper, we investigate a two-way relaying power line communication (PLC) network with analog network coding. We focus on the analysis of the system outage probability, symbol error rate, and average capacity. Specifically, we first derive the probability density function (PDF) of the received signal-to-noise ratio (SNR) with a closed form, by exploiting the statistical properties of the PLC channel. Then with the help of this PDF, we develop the outage probability, symbol error rate, and average capacity with closed forms, based on the Hermite polynomial. Simulations show that the derived analytical results are consistent with those by Monte Carlo simulation.

关键词： Power line communication     Outage probability     Symbol error rate     Signal attenuation    

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An improved artificial bee colony algorithm with MaxTF heuristic rule for two-sided assembly line balancing

Xiaokun DUAN, Bo WU, Youmin HU, Jie LIU, Jing XIONG

《机械工程前沿（英文）》 2019年 第14卷 第2期   页码 241-253 doi: 10.1007/s11465-018-0518-6

摘要： Two-sided assembly line is usually used for the assembly of large products such as cars, buses, and trucks. With the development of technical progress, the assembly line needs to be reconfigured and the cycle time of the line should be optimized to satisfy the new assembly process. Two-sided assembly line balancing with the objective of minimizing the cycle time is called TALBP-2. This paper proposes an improved artificial bee colony (IABC) algorithm with the MaxTF heuristic rule. In the heuristic initialization process, the MaxTF rule defines a new task’s priority weight. On the basis of priority weight, the assignment of tasks is reasonable and the quality of an initial solution is high. In the IABC algorithm, two neighborhood strategies are embedded to balance the exploitation and exploration abilities of the algorithm. The employed bees and onlooker bees produce neighboring solutions in different promising regions to accelerate the convergence rate. Furthermore, a well-designed random strategy of scout bees is developed to escape local optima. The experimental results demonstrate that the proposed MaxTF rule performs better than other heuristic rules, as it can find the best solution for all the 10 test cases. A comparison of the IABC algorithm and other algorithms proves the effectiveness of the proposed IABC algorithm. The results also denote that the IABC algorithm is efficient and stable in minimizing the cycle time for the TALBP-2, and it can find 20 new best solutions among 25 large-sized problem cases.

关键词： two-sided assembly line balancing problem     artificial bee colony algorithm     heuristic rules     time boundary    

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Closed surface modeling with helical line measurement data

LI Ruqiong, LI Guangbu, WANG Yuhan

《机械工程前沿（英文）》 2007年 第2卷 第1期   页码 72-76 doi: 10.1007/s11465-007-0012-z

摘要： Models for surface modeling of free-form surface and massive data points are becoming an important feature in commercial computer aided design/computer-aided manufacturing software. However, there are many problems to be solved in this area, especially for closed free-form surface modeling. This article presents an effective method for cloud data closed surface modeling from asynchronous profile modeling measurement. It includes three steps: first, the cloud data are preprocessed for smoothing; second, a helical line is segmented to form triangle meshes; and third, Bezier surface patches are created over a triangle mesh and trimmed to shape on an entire surface. In the end, an illustrative example of shoe last surface modeling is given to show the availability of this method.

关键词： free-form surface     helical     triangle     design/computer-aided manufacturing     effective    

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Hepatitis B virus X protein upregulates tumor necrosis factor-α expression of rat mesangial cell line

Hong-Zhu LU MD, Dan LIU BM, Qi-Hong FAN BM, Jian-Hua ZHOU MD,

《医学前沿（英文）》 2010年 第4卷 第1期   页码 106-111 doi: 10.1007/s11684-010-0004-y

摘要： Hepatitis B virus X protein (HBx), a 17-kd protein encoded by X gene of hepatitis B virus (HBV), has been shown to function as a transcriptional trans-activator of a variety of viral and cellular promoter/enhancer elements. The aim of the study is to investigate the extracellular regulated protein kinases (ERKs) pathway of HBx on glomerular mesangial cell (GMC) proliferation and tumor necrosis factor-α (TNF-α) expression. The HBV X gene was amplified by polymerase chain reaction (PCR), inserted into the eukaryotic expression vector pCI-neo and confirmed by restriction endonuclease digestion and sequence analysis. PCI-neo containing HBV X gene (pCI-neo-X) was then transfected into cultured GMC line via liposome. GMC proliferation, TNF-α and its mRNA expression were compared in the condition of with or without U0126 in culture media. HBx, ERK and p-ERK expression in GMCs was assessed by Western blotting. TNF-α mRNA expression was assessed by semi-quantitative reverse transcription-PCR (RT-PCR). TNF-α level in supernatants was measured by ELISA. GMC proliferation was detected by 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT) kit. The results showed that HBx expression was found in transfected GMCs and became prominent at 36th and 48th h after transfection whether with or without U0126 in culture media. TNF-α mRNA expression was significantly decreased in U0126 group compared with U0126-free group. TNF-α levels in supernatants in PCI-neo-X transfection without U0126 group were (189.0±18.1) and (172.3±24.3) pg/mL at 36th and 48th h after transfection, respectively. In contrast, TNF-α levels in supernatants with U0126 were (65.6±11.6) and (84.0±24.6) pg/mL at 36th and 48th h, respectively. The TNF-α levels in the latter groups were significantly lower than those in the former groups (<0.05). GMCs proliferation was also lower in added U0126 group at 36th and 48th h after transfection. From above, we can conclude that HBx could induce GMC proliferation and increase TNF-α mRNA expression and its protein production. HBx upregulates TNF-α expression and induces cell proliferation of GMC line partly through ERK signal transduction pathway.

关键词： hepatitis B virus     X gene     glomerular mesangial cell line     extracellular regulated protein kinases     tumor necrosis factor-α    

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标题 作者 时间 类型 操作

FUNCTIONAL GAIN OF FRUIT NETTED-CRACKING IN AN INTROGRESSION LINE OF TOMATO WITH HIGHER EXPRESSION OF

期刊论文

FUNCTIONAL GAIN OF FRUIT NETTED-CRACKING IN AN INTROGRESSION LINE OF TOMATO WITH HIGHER EXPRESSION OF

Chunli ZHANG, Taotao WANG, Jing LI, Danqiu ZHANG, Qingmin XIE, Shoaib MUNIR, Jie YE, Hanxia LI, Yongen LU, Changxian YANG, Bo OUYANG, Yuyang ZHANG, Junhong ZHANG, Zhibiao YE

期刊论文

Acoustic fault signal extraction via the line-defect phononic crystals

期刊论文

Calculations of narrow-band transimissities and the Planck mean absorption coefficients of real gases using line-by-line

Huaqiang CHU, Mingyan GU, Huaichun ZHOU, Fengshan LIU

期刊论文

Pareto lexicographic α-robust approach and its application in robust multi objective assembly line balancing

null

期刊论文

Choosing configurations of transmission line tower grounding by back flashover probability value

Dmitry KUKLIN

期刊论文

A fast compound direct iterative algorithm for solving transient line contact elastohydrodynamic lubrication

null

期刊论文

A novel light fluctuation spectrum method for in-line particle sizing

Shouxuan QIN, Xiaoshu CAI, Li MA

期刊论文

A naive optimization method for multi-line systems with alternative machines

Weichang KONG, Fei QIAO, Qidi WU

期刊论文

Establishment and drug sensitivity evaluation of murine ascites hepatocarcinoma cell line with high lymphatic

Hongying ZHANG, Jianwu TANG, Wenting ZHU, Chunxiu HU, Guowang XU

期刊论文

基于灰色模糊数的公交线网优化研究

邓卫,胡启洲

期刊论文

Performance analysis for a two-way relaying power line networkwith analog network coding

Yu-wen QIAN,Meng TIAN,Xue JIANG,Hua-ju SONG,Feng SHU,Jun LI

期刊论文

An improved artificial bee colony algorithm with MaxTF heuristic rule for two-sided assembly line balancing

Xiaokun DUAN, Bo WU, Youmin HU, Jie LIU, Jing XIONG

期刊论文

Closed surface modeling with helical line measurement data

LI Ruqiong, LI Guangbu, WANG Yuhan

期刊论文

Hepatitis B virus X protein upregulates tumor necrosis factor-α expression of rat mesangial cell line

Hong-Zhu LU MD, Dan LIU BM, Qi-Hong FAN BM, Jian-Hua ZHOU MD,

期刊论文